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KMID : 0383819780250020057
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Tuberculosis and Respiratory Diseases
1978 Volume.25 No. 2 p.57 ~ p.62
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A Study on Newly Formulated Mycobacterial Media and Their Application in Rapid Diagnosis of Chronic Mycobacterial Diseases
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유준(柳駿)/Joon LEW
이영남(李泳南)/김성광(金聖光)/Young Nam LEE/Sung Kwang KIM
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Abstract
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We made attempts to formulate sin)pie media supporting better growth of
mycobacterial species and to evaluate feasibility of the media using in rapid diagnosis of
the chromo mycobacterial diseases. One of the media formulated is a phosphate buffered
medium with 0.1% hyaluronic acid and 6.0% bovine serum albumin in addition to 3.0%
gycero1.
This medium was orginally formulated by Skinsnes' group, but we made slight
modification. The other is a semisynthetic medium made of umbilical cord extract
supplemented with 10% sheep serum as a final concentration. Mycobacterial species
employed in the experiments gave better growth yield in the newly formulated media
than the conventional Dubos liquid medium. We also checked pathogenicity of M.
tuberculosis H37Rv grown in the different media to mouse. Tubercle bacilli
grown in the Dubos liquid medium showed more potent pathogenicity to mouse than
those grown in the newly formulated media.
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